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sucrose counter selectable pre112  (Addgene inc)
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Addgene inc sucrose counter selectable pre112
Fig. 1 Schematic map of suicide vectors. A All suicide vectors were derived from pHY093 <t>(pRE112-Cm-GFP)</t> or pHY094 (pRE112-Kan-GFP). B Two homologous arms, i.e., HA_up and HA_down were cloned from E. coli W3110 genome and fused together by fusion PCR. The fused homologous arms replaced the GFP cassette in pHY093 (pRE112-Cm-GFP), resulting in pHY095 (pRE112-Cm-ΔOAg). C the viaB_locus was cloned from S. Typhi genome. The linearized pHY095 (pRE112-Cm-ΔOAg) vector and viaB_locus were assembled in vitro, resulting in pHY129 (pRE112-Cm-ΔOAg::viaB locus). D A cassette of FRT-flanked chloramphenicol resistance gene was cloned from pCP3, and cloned into the middle of homologous arms, resulting in pHY099 (pRE112-Km-ΔOAg::Cm_FRT2). E The OAgST O-antigen gene cluster was cloned from S. Typhimurium genome via Long-range PCR. The linearized pHY099 (pRE112-Km-ΔOAg::Cm_FRT2) vector and OAgST were assembly in vitro, resulting in pHY100 (pRE112-Km-ΔOAg::OAgST-Cm_FRT2). F The OAgSE O-antigen gene cluster was cloned from S. Enteritidis genome via Long-range PCR. The linearized pHY099 (pRE112-Km-ΔOAg::Cm_FRT2) vector and OAgSE were assembly in vitro, resulting in pHY101 (pRE112-Km-ΔOAg::OAgSE-Cm_FRT2). G pHY102 (pRE112-Km-ΔOAg::OAgSA-Cm_FRT2) was derived from pHY101 by deliberately deleting the tyv gene
Sucrose Counter Selectable Pre112, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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kibdelosporangium aridum dsm 43828  (DSMZ)
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DSMZ kibdelosporangium aridum dsm 43828
Fig. 1 Schematic map of suicide vectors. A All suicide vectors were derived from pHY093 <t>(pRE112-Cm-GFP)</t> or pHY094 (pRE112-Kan-GFP). B Two homologous arms, i.e., HA_up and HA_down were cloned from E. coli W3110 genome and fused together by fusion PCR. The fused homologous arms replaced the GFP cassette in pHY093 (pRE112-Cm-GFP), resulting in pHY095 (pRE112-Cm-ΔOAg). C the viaB_locus was cloned from S. Typhi genome. The linearized pHY095 (pRE112-Cm-ΔOAg) vector and viaB_locus were assembled in vitro, resulting in pHY129 (pRE112-Cm-ΔOAg::viaB locus). D A cassette of FRT-flanked chloramphenicol resistance gene was cloned from pCP3, and cloned into the middle of homologous arms, resulting in pHY099 (pRE112-Km-ΔOAg::Cm_FRT2). E The OAgST O-antigen gene cluster was cloned from S. Typhimurium genome via Long-range PCR. The linearized pHY099 (pRE112-Km-ΔOAg::Cm_FRT2) vector and OAgST were assembly in vitro, resulting in pHY100 (pRE112-Km-ΔOAg::OAgST-Cm_FRT2). F The OAgSE O-antigen gene cluster was cloned from S. Enteritidis genome via Long-range PCR. The linearized pHY099 (pRE112-Km-ΔOAg::Cm_FRT2) vector and OAgSE were assembly in vitro, resulting in pHY101 (pRE112-Km-ΔOAg::OAgSE-Cm_FRT2). G pHY102 (pRE112-Km-ΔOAg::OAgSA-Cm_FRT2) was derived from pHY101 by deliberately deleting the tyv gene
Kibdelosporangium Aridum Dsm 43828, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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stock n a pre112 δrecf plac daca aca  (Addgene inc)
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Addgene inc stock n a pre112 δrecf plac daca aca
Fig. 1 Schematic map of suicide vectors. A All suicide vectors were derived from pHY093 <t>(pRE112-Cm-GFP)</t> or pHY094 (pRE112-Kan-GFP). B Two homologous arms, i.e., HA_up and HA_down were cloned from E. coli W3110 genome and fused together by fusion PCR. The fused homologous arms replaced the GFP cassette in pHY093 (pRE112-Cm-GFP), resulting in pHY095 (pRE112-Cm-ΔOAg). C the viaB_locus was cloned from S. Typhi genome. The linearized pHY095 (pRE112-Cm-ΔOAg) vector and viaB_locus were assembled in vitro, resulting in pHY129 (pRE112-Cm-ΔOAg::viaB locus). D A cassette of FRT-flanked chloramphenicol resistance gene was cloned from pCP3, and cloned into the middle of homologous arms, resulting in pHY099 (pRE112-Km-ΔOAg::Cm_FRT2). E The OAgST O-antigen gene cluster was cloned from S. Typhimurium genome via Long-range PCR. The linearized pHY099 (pRE112-Km-ΔOAg::Cm_FRT2) vector and OAgST were assembly in vitro, resulting in pHY100 (pRE112-Km-ΔOAg::OAgST-Cm_FRT2). F The OAgSE O-antigen gene cluster was cloned from S. Enteritidis genome via Long-range PCR. The linearized pHY099 (pRE112-Km-ΔOAg::Cm_FRT2) vector and OAgSE were assembly in vitro, resulting in pHY101 (pRE112-Km-ΔOAg::OAgSE-Cm_FRT2). G pHY102 (pRE112-Km-ΔOAg::OAgSA-Cm_FRT2) was derived from pHY101 by deliberately deleting the tyv gene
Stock N A Pre112 δrecf Plac Daca Aca, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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stock n a pya3736 pre112 δasda33 lab  (Addgene inc)
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Addgene inc stock n a pya3736 pre112 δasda33 lab
Fig. 1 Schematic map of suicide vectors. A All suicide vectors were derived from pHY093 <t>(pRE112-Cm-GFP)</t> or pHY094 (pRE112-Kan-GFP). B Two homologous arms, i.e., HA_up and HA_down were cloned from E. coli W3110 genome and fused together by fusion PCR. The fused homologous arms replaced the GFP cassette in pHY093 (pRE112-Cm-GFP), resulting in pHY095 (pRE112-Cm-ΔOAg). C the viaB_locus was cloned from S. Typhi genome. The linearized pHY095 (pRE112-Cm-ΔOAg) vector and viaB_locus were assembled in vitro, resulting in pHY129 (pRE112-Cm-ΔOAg::viaB locus). D A cassette of FRT-flanked chloramphenicol resistance gene was cloned from pCP3, and cloned into the middle of homologous arms, resulting in pHY099 (pRE112-Km-ΔOAg::Cm_FRT2). E The OAgST O-antigen gene cluster was cloned from S. Typhimurium genome via Long-range PCR. The linearized pHY099 (pRE112-Km-ΔOAg::Cm_FRT2) vector and OAgST were assembly in vitro, resulting in pHY100 (pRE112-Km-ΔOAg::OAgST-Cm_FRT2). F The OAgSE O-antigen gene cluster was cloned from S. Enteritidis genome via Long-range PCR. The linearized pHY099 (pRE112-Km-ΔOAg::Cm_FRT2) vector and OAgSE were assembly in vitro, resulting in pHY101 (pRE112-Km-ΔOAg::OAgSE-Cm_FRT2). G pHY102 (pRE112-Km-ΔOAg::OAgSA-Cm_FRT2) was derived from pHY101 by deliberately deleting the tyv gene
Stock N A Pya3736 Pre112 δasda33 Lab, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pre112  (Addgene inc)
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Addgene inc pre112
Fig. 1 Schematic map of suicide vectors. A All suicide vectors were derived from pHY093 <t>(pRE112-Cm-GFP)</t> or pHY094 (pRE112-Kan-GFP). B Two homologous arms, i.e., HA_up and HA_down were cloned from E. coli W3110 genome and fused together by fusion PCR. The fused homologous arms replaced the GFP cassette in pHY093 (pRE112-Cm-GFP), resulting in pHY095 (pRE112-Cm-ΔOAg). C the viaB_locus was cloned from S. Typhi genome. The linearized pHY095 (pRE112-Cm-ΔOAg) vector and viaB_locus were assembled in vitro, resulting in pHY129 (pRE112-Cm-ΔOAg::viaB locus). D A cassette of FRT-flanked chloramphenicol resistance gene was cloned from pCP3, and cloned into the middle of homologous arms, resulting in pHY099 (pRE112-Km-ΔOAg::Cm_FRT2). E The OAgST O-antigen gene cluster was cloned from S. Typhimurium genome via Long-range PCR. The linearized pHY099 (pRE112-Km-ΔOAg::Cm_FRT2) vector and OAgST were assembly in vitro, resulting in pHY100 (pRE112-Km-ΔOAg::OAgST-Cm_FRT2). F The OAgSE O-antigen gene cluster was cloned from S. Enteritidis genome via Long-range PCR. The linearized pHY099 (pRE112-Km-ΔOAg::Cm_FRT2) vector and OAgSE were assembly in vitro, resulting in pHY101 (pRE112-Km-ΔOAg::OAgSE-Cm_FRT2). G pHY102 (pRE112-Km-ΔOAg::OAgSA-Cm_FRT2) was derived from pHY101 by deliberately deleting the tyv gene
Pre112, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e coli  (Addgene inc)
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Addgene inc e coli
Fig. 1 Schematic map of suicide vectors. A All suicide vectors were derived from pHY093 <t>(pRE112-Cm-GFP)</t> or pHY094 (pRE112-Kan-GFP). B Two homologous arms, i.e., HA_up and HA_down were cloned from E. coli W3110 genome and fused together by fusion PCR. The fused homologous arms replaced the GFP cassette in pHY093 (pRE112-Cm-GFP), resulting in pHY095 (pRE112-Cm-ΔOAg). C the viaB_locus was cloned from S. Typhi genome. The linearized pHY095 (pRE112-Cm-ΔOAg) vector and viaB_locus were assembled in vitro, resulting in pHY129 (pRE112-Cm-ΔOAg::viaB locus). D A cassette of FRT-flanked chloramphenicol resistance gene was cloned from pCP3, and cloned into the middle of homologous arms, resulting in pHY099 (pRE112-Km-ΔOAg::Cm_FRT2). E The OAgST O-antigen gene cluster was cloned from S. Typhimurium genome via Long-range PCR. The linearized pHY099 (pRE112-Km-ΔOAg::Cm_FRT2) vector and OAgST were assembly in vitro, resulting in pHY100 (pRE112-Km-ΔOAg::OAgST-Cm_FRT2). F The OAgSE O-antigen gene cluster was cloned from S. Enteritidis genome via Long-range PCR. The linearized pHY099 (pRE112-Km-ΔOAg::Cm_FRT2) vector and OAgSE were assembly in vitro, resulting in pHY101 (pRE112-Km-ΔOAg::OAgSE-Cm_FRT2). G pHY102 (pRE112-Km-ΔOAg::OAgSA-Cm_FRT2) was derived from pHY101 by deliberately deleting the tyv gene
E Coli, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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acknowledgments plasmid pre112  (Addgene inc)
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Addgene inc acknowledgments plasmid pre112
Fig. 1 Schematic map of suicide vectors. A All suicide vectors were derived from pHY093 <t>(pRE112-Cm-GFP)</t> or pHY094 (pRE112-Kan-GFP). B Two homologous arms, i.e., HA_up and HA_down were cloned from E. coli W3110 genome and fused together by fusion PCR. The fused homologous arms replaced the GFP cassette in pHY093 (pRE112-Cm-GFP), resulting in pHY095 (pRE112-Cm-ΔOAg). C the viaB_locus was cloned from S. Typhi genome. The linearized pHY095 (pRE112-Cm-ΔOAg) vector and viaB_locus were assembled in vitro, resulting in pHY129 (pRE112-Cm-ΔOAg::viaB locus). D A cassette of FRT-flanked chloramphenicol resistance gene was cloned from pCP3, and cloned into the middle of homologous arms, resulting in pHY099 (pRE112-Km-ΔOAg::Cm_FRT2). E The OAgST O-antigen gene cluster was cloned from S. Typhimurium genome via Long-range PCR. The linearized pHY099 (pRE112-Km-ΔOAg::Cm_FRT2) vector and OAgST were assembly in vitro, resulting in pHY100 (pRE112-Km-ΔOAg::OAgST-Cm_FRT2). F The OAgSE O-antigen gene cluster was cloned from S. Enteritidis genome via Long-range PCR. The linearized pHY099 (pRE112-Km-ΔOAg::Cm_FRT2) vector and OAgSE were assembly in vitro, resulting in pHY101 (pRE112-Km-ΔOAg::OAgSE-Cm_FRT2). G pHY102 (pRE112-Km-ΔOAg::OAgSA-Cm_FRT2) was derived from pHY101 by deliberately deleting the tyv gene
Acknowledgments Plasmid Pre112, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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suicide plasmid pre112  (Addgene inc)
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Addgene inc suicide plasmid pre112
Fig. 1 Schematic map of suicide vectors. A All suicide vectors were derived from pHY093 <t>(pRE112-Cm-GFP)</t> or pHY094 (pRE112-Kan-GFP). B Two homologous arms, i.e., HA_up and HA_down were cloned from E. coli W3110 genome and fused together by fusion PCR. The fused homologous arms replaced the GFP cassette in pHY093 (pRE112-Cm-GFP), resulting in pHY095 (pRE112-Cm-ΔOAg). C the viaB_locus was cloned from S. Typhi genome. The linearized pHY095 (pRE112-Cm-ΔOAg) vector and viaB_locus were assembled in vitro, resulting in pHY129 (pRE112-Cm-ΔOAg::viaB locus). D A cassette of FRT-flanked chloramphenicol resistance gene was cloned from pCP3, and cloned into the middle of homologous arms, resulting in pHY099 (pRE112-Km-ΔOAg::Cm_FRT2). E The OAgST O-antigen gene cluster was cloned from S. Typhimurium genome via Long-range PCR. The linearized pHY099 (pRE112-Km-ΔOAg::Cm_FRT2) vector and OAgST were assembly in vitro, resulting in pHY100 (pRE112-Km-ΔOAg::OAgST-Cm_FRT2). F The OAgSE O-antigen gene cluster was cloned from S. Enteritidis genome via Long-range PCR. The linearized pHY099 (pRE112-Km-ΔOAg::Cm_FRT2) vector and OAgSE were assembly in vitro, resulting in pHY101 (pRE112-Km-ΔOAg::OAgSE-Cm_FRT2). G pHY102 (pRE112-Km-ΔOAg::OAgSA-Cm_FRT2) was derived from pHY101 by deliberately deleting the tyv gene
Suicide Plasmid Pre112, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 1 Schematic map of suicide vectors. A All suicide vectors were derived from pHY093 (pRE112-Cm-GFP) or pHY094 (pRE112-Kan-GFP). B Two homologous arms, i.e., HA_up and HA_down were cloned from E. coli W3110 genome and fused together by fusion PCR. The fused homologous arms replaced the GFP cassette in pHY093 (pRE112-Cm-GFP), resulting in pHY095 (pRE112-Cm-ΔOAg). C the viaB_locus was cloned from S. Typhi genome. The linearized pHY095 (pRE112-Cm-ΔOAg) vector and viaB_locus were assembled in vitro, resulting in pHY129 (pRE112-Cm-ΔOAg::viaB locus). D A cassette of FRT-flanked chloramphenicol resistance gene was cloned from pCP3, and cloned into the middle of homologous arms, resulting in pHY099 (pRE112-Km-ΔOAg::Cm_FRT2). E The OAgST O-antigen gene cluster was cloned from S. Typhimurium genome via Long-range PCR. The linearized pHY099 (pRE112-Km-ΔOAg::Cm_FRT2) vector and OAgST were assembly in vitro, resulting in pHY100 (pRE112-Km-ΔOAg::OAgST-Cm_FRT2). F The OAgSE O-antigen gene cluster was cloned from S. Enteritidis genome via Long-range PCR. The linearized pHY099 (pRE112-Km-ΔOAg::Cm_FRT2) vector and OAgSE were assembly in vitro, resulting in pHY101 (pRE112-Km-ΔOAg::OAgSE-Cm_FRT2). G pHY102 (pRE112-Km-ΔOAg::OAgSA-Cm_FRT2) was derived from pHY101 by deliberately deleting the tyv gene

Journal: Microbial cell factories

Article Title: Genetic engineering of E. coli K-12 for heterologous carbohydrate antigen production.

doi: 10.1186/s12934-025-02749-2

Figure Lengend Snippet: Fig. 1 Schematic map of suicide vectors. A All suicide vectors were derived from pHY093 (pRE112-Cm-GFP) or pHY094 (pRE112-Kan-GFP). B Two homologous arms, i.e., HA_up and HA_down were cloned from E. coli W3110 genome and fused together by fusion PCR. The fused homologous arms replaced the GFP cassette in pHY093 (pRE112-Cm-GFP), resulting in pHY095 (pRE112-Cm-ΔOAg). C the viaB_locus was cloned from S. Typhi genome. The linearized pHY095 (pRE112-Cm-ΔOAg) vector and viaB_locus were assembled in vitro, resulting in pHY129 (pRE112-Cm-ΔOAg::viaB locus). D A cassette of FRT-flanked chloramphenicol resistance gene was cloned from pCP3, and cloned into the middle of homologous arms, resulting in pHY099 (pRE112-Km-ΔOAg::Cm_FRT2). E The OAgST O-antigen gene cluster was cloned from S. Typhimurium genome via Long-range PCR. The linearized pHY099 (pRE112-Km-ΔOAg::Cm_FRT2) vector and OAgST were assembly in vitro, resulting in pHY100 (pRE112-Km-ΔOAg::OAgST-Cm_FRT2). F The OAgSE O-antigen gene cluster was cloned from S. Enteritidis genome via Long-range PCR. The linearized pHY099 (pRE112-Km-ΔOAg::Cm_FRT2) vector and OAgSE were assembly in vitro, resulting in pHY101 (pRE112-Km-ΔOAg::OAgSE-Cm_FRT2). G pHY102 (pRE112-Km-ΔOAg::OAgSA-Cm_FRT2) was derived from pHY101 by deliberately deleting the tyv gene

Article Snippet: The gene cluster insertion mutations on the E. coli chromosome were constructed using the sucrose counter-selectable pRE112 (addgene, plasmid #43,828) suicide vectors [24].

Techniques: Derivative Assay, Clone Assay, Plasmid Preparation, In Vitro, Long Range PCR